Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
AdipoGen Trishydroxymethyl aminometha
Chemical. CAS 77-86-1. Formula C4H11NO3. MW 121.14. Synthetic. A high quality buffer used in the range of physiological pH 7-9. Widely used as a biological buffer or as a component of buffer formulations. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms pH 7.0-9.0. Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
Zymo Research Corporation ZymoPURE Binding Buffer, 210 ml
ZymoPURE Binding Buffer is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Biotium TBE Buffer, 5X
5X TBE (Tris-Borate-EDTA) is a concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis. 1X solution: 89 mM Tris,89 mM boric acid, 2 mM EDTA. Store at room temperature.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.6 - MB-003
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-5000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 5 Kilograms
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Bioworld Tris-Triton Buffer, 500 mL
Tris-Triton bufferis the idealbuffer with detergent required to release membrane- or cytoskeleton- bound proteins.Contains: 10mM Tris (pH 7.4), 100mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 10% Glycerol and 0.1% SDSAppearance: Liquid
Teknova Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Teknova 1M Tris-HCl, pH 8.0 Solution. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Tris-EDTA Buffer, TE (20X, pH 8.0) - 250ml
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of a 20X concentration of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allows pH regulation within the range of 7.5-8.5. It is widely used often for DNA and RNA gel electrophoresis, and also for DNA automated sequencing.
Medchemexpress LLC P62-mediated mitopha 50mg | 1809031-84-2 | 392.15 | C14H9IN4O2 | 50 MG
P62-mediated mitophagy inducer is a small-molecule activator of p62-dependent mitophagy used in research to stimulate mitochondrial autophagy without recruiting Parkin or collapsing mitochondrial membrane potential.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Promotes mitochondrial autophagy while preserving mitochondrial membrane potential.
- Suitable for autophagy and mitophagy research applications.
- Powder form with defined cold-storage stability for long-term storage.
- Available in small research pack sizes for laboratory use.
- Molecular formula C14H9IN4O2; molecular weight 392.15 g·mol⁻¹.
Abcam 10 x RIPA Buffer, 15ML
10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins
The product is subject to the following: Abcam Restricted Use Statement
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X) - 1L
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
![Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660920.jpg-250.jpg)
Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 L
TAE 10X Solution (TRIS Acetate EDTA) is a concentrated stock buffer solution used for nucleic acid electrophoresis and various molecular biology techniques. This 1-liter bottle provides a 10X stock solution that can be easily diluted to a working concentration of 1X by adding the appropriate volume of water. TAE buffer is preferred for its low ionic strength, which helps reduce background noise and allows for better resolution of nucleic acid fragments during gel electrophoresis. The inclusion of EDTA in the buffer helps protect DNA and RNA from degradation by chelating metal ions that may cause hydrolysis, making it an essential tool for safe, effective molecular analysis.
Boston Bioproducts Inc 10mM Tris-HCl pH 8 1L
Tris-HCl Buffer (10 mM pH 8 0) - 1L (For Research Use and Further Manufacturing Only)