Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC Annexin V-APC/PI Apoptosis Kit 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (APC) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes Additionally this kit can be paired with the nuclear dye propidium iodide (PI) PI specifically binds to double-stranded DNA emitting red fluorescence upon binding but it cannot normally permeate intact cell membranes However PI can penetrate compromised cellular membranes in late-stage apoptotic and dead cells staining nuclei Therefore combined staining with Annexin V-APC and PI allows
Sigma Aldrich Fine Chemicals Biosciences Iscoves Modified Dulbeccos
ISCOVES Iscoves Modified Dulbeccos
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Seracare Life Sciences Inc 10X PHOSPHATE BUFFERED SALINE
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled.
Apexbio Technology LLC Annexin V-PE Apoptosis Kit 20tests
Annexin V-PE Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Alternatively you can directly use our ready-to-use dual-staining kits Annexin V-PE/7-AAD Apoptosis Kit ( K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ PBS 10X, pH 7.2, without calcium, magnesium, 500mL, 500ml, 10/case
CellPro™ PBS, 10X, pH7.2, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
![Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660867.jpg-250.jpg)
Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram
TES, Sodium Salt, is a high-purity buffering agent available in a 1-kilogram package. This tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid sodium salt is widely used in biochemical and molecular biology research for maintaining stable pH conditions in solutions. TES buffers are particularly effective in biological and clinical applications where precise pH control is essential. The high purity of this reagent makes it suitable for sensitive experiments, including enzyme assays, cell culture, and PCR reactions. TES buffers are used to stabilize proteins, DNA, and other biomolecules, ensuring consistent and reliable results in a variety of laboratory protocols.
![Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661333.jpg-250.jpg)
Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 25 G
HEPPSO (N-(2 Hydroxyethyl) piperazine N'-(2 hydroxypropanesulfonic acid)) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in laboratory applications for maintaining pH stability in aqueous solutions. HEPPSO is ideal for use in biochemical research, including enzyme assays, protein purification, and other experiments requiring precise pH regulation. It helps maintain a stable pH environment in various biological assays, making it essential in molecular biology, cell culture, and electrophoresis. The 25g size makes it perfect for smaller-scale laboratory experiments where reliable buffering capacity is needed.
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 100G Sto
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 100G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 100G
Chem-Impex International, Inc. MOPS sodium salt | 71119-22-7 | MFCD00064350 | 1KG
MOPS sodium salt, 71119-22-7, MFCD00064350, 1KG
Teknova 20mM HEPES, 150mM Sodium Chloride, pH 7.0. 1000mL. Endotoxin Tested, Sterile. 8 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660391.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in molecular biology, biochemistry, and analytical chemistry for maintaining stable pH levels in low pH environments. DIPSO is ideal for enzyme assays, protein research, and chromatography, where maintaining a precise pH is critical. It is also useful in protein purification and electrophoresis, providing consistent and reliable results. The 25g size is perfect for smaller scale experiments or routine laboratory applications where a high-quality buffering solution is necessary for pH regulation.